Earticle

초록

영어

The experimental manipulation of protooncogenes and their gene products is a valuable research tool for the study of human neoplasia. In this study, the recently identified human cervical cancer protooncogene (HccR-2) was expressed in transgenic mice under the control of the tetracycline regulatory system. The phenotype observed was similar in many respects to human chronic neutrophilic leukemia (CNL). Thus, the HccR-2 transgenic mouse model is important not only for investigating the biological properties of the HccR-2 protooncogene in vivo, but also for analyzing the mechanisms involved in the progression of CNL.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Production of Transgenic Mice
  PCR Analysis
  Southern Blot Hybridization Analysis
  RNA Analysis by Reverse Transcriptase-PCR (RT-PCR)
  Western Blot Hybridization Analysis
  Morphologic and Histologic Analyses
  Statistical Analysis
 RESULTS
  Generation of the Transgenic Mice Expressing HccR-2
  Peripheral Blood and Bone Marrow Features
  Development of Chronic Neutrophilic Leukemia (CNL)
  Leukemic Transgenic Mice were Susceptible to Infection
 DISCUSSION
 REFERENCES

키워드

저자

• Byoung Boo Seo [ Dept. of Animal Resources, College of Life & Environmental Science, Daegu University ]
• Sung-Hun Min [ Dept. of Biotechnology, College of Engineering, Daegu University ]
• Eun Ji Lee [ Dept. of Obstetrics and Gynecololgy, Yeung nam University ]
• Zae Young Ryoo [ School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University ]
• Humdai Park [ Dept. of Biotechnology, College of Engineering, Daegu University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636