Earticle

초록

영어

The experimental manipulation of protooncogenes and their gene products is a valuable research tool for the study of human neoplasia. In this study, the recently identified human cervical cancer protooncogene (HccR-2) was expressed in transgenic mice under the control of the tetracycline regulatory system. Mice expressing the HccR-2 transgene showed an altered myeloid development characterized by an increased percentage of mature and band-form neutrophils in the peripheral blood, liver and spleen. This phenotype is similar to human chronic neutrophilic leukemia (CNL) in many ways, which is a rare chronic myeloproliferative disorder (CMD) that presents as a sustained leukocytosis of mature neutrophils with a few or no circulating immature granulocytes, an absence of peripheral blood monocytosis, basophilia, or eosinophilia, and an infiltration of neutrophils into the liver, spleen and kidney. Thus, the HccR-2 transgenic mouse model is imperative not only for investigating the biological properties of the HccR-2 protooncogene in vivo, but also for analyzing the mechanisms involved in the progression of CNL.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Generation of Transgenic Mice
  PCR Analysis
  Southern Blot Hybridization Analysis
  RNA Analysis by Reverse Transcriptase-PCR (RTPCR)
  Western Blot Hybridization Analysis
  Morphologic and Histologic Analyses
  Statistical Analysis
 RESULTS
  Production of the Transgenic Mice Expressing Hcc-R-2
  Clinical and Laboratory Diagnosis
  Development of Chronic Neutrophilic Leukemia(CNL)
 DISCUSSION
 REFERENCES

키워드

저자

• Byoung Boo Seo [ Dept. of Animal Resources, College of Life & Environmental Science, Daegu University ]
• Humdai Park [ Dept. of Biotechnology, College of Engineering, Daegu University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636