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Localization of Cyclin B and Erk1/2 in Ovine Oocytes and MPF and MAPK Activities in Cytoplast and Karyoplast following Enucleation

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 35 No 4 (2011.12)바로가기
  • 페이지
    pp.407-414
  • 저자
    Joon-Hee Lee, Keith H. S. Campbell
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A163574

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원문정보

초록

영어
The development of embryos reconstructed by somatic cell nuclear transfer (SCNT) is dependent upon numerous factors. Central to development is the quality and developmental competence of the recipient cytoplast and the type of the donor nucleus. Typically metaphase of the second meiotic division (MII) has become the cytoplast of choice. Production of a cytoplast requires removal of the recipient genetic material, however, it may remove proteins which are essential for development or reduce the levels of cytoplasmic proteins to influence subsequent reprogramming of the donor nucleus. In this study, enucleation at MII did not affect the activities of either MPF or MAPK kinases.
Immunocytochemical staining showed that both Cyclin B1 (MPF) and Erk1/2 (MAPK) were associated with the meiotic spindle of AI/TI oocytes with little staining in the cytoplasm, however, at MII association of both proteins with the spindle had reduced and a greater degree of cytoplasmic distribution was observed. The analysis of oocyte proteins removed during enucleation is a difficult approach to the identification of factors which may be depleted in the cytoplast.
This is primarily due to the large numbers of aspirated karyoplasts which would be required for the analysis.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Collection and Culture of Sheep Oocytes
  Assessment of MPF and MAPK Activities
  Immunocytochemistry
  Enucleation
  Electrophoretic Separation of Proteins
  Statistical Analysis
 RESULTS
  MPF and MAPK Activities at Different Stages of Maturation
  Localization of Cyclin B1 and Erk1/2 in Ovine Oocytes
  MPF and MAPK Activities of Cytoplasts and KaryoplastsFollowing Enucleation
  Determination of Proteins Depleted from Oocytes Following Enucleation
 DISCUSSION
 REFERENCES

키워드

Somatic cell nuclear transfer MPF MAPK Immunocytochemical staining Enucleation

저자

  • Joon-Hee Lee [ Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Division of Animal Physiology, School of Biosciences, The University of Nottingham, Sutton Bonington,Institute of Agriculture & Life Science, College of Agriculture and Life Sciences, Gyeongsang National University ] Corresponding author
  • Keith H. S. Campbell [ Division of Animal Physiology, School of Biosciences, The University of Nottingham ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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