Earticle

초록

영어

Zinc protoporphyrin (ZnPP) is produced endogenously during heme metabolism and treated in cells as a heme oxygenase inhibitor. In the present study, the effects of ZnPP on the color response of 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, a commonly-used method for analyzing cell viability, were investigated. ZnPP induced rapid decolorizaion of MTT formazan under light; the degradation rates were 10- and 20- folds faster in the presence of 5 and 10 μM ZnPP, respectively. Methylene blue (MB), another type of photosensitizer, also accelerated degradation of formazan under light. Butylated hyroxytoluene did not inhibit ZnPP- or MB-induced formazan degradation. The color degradation of formazan dye was signficantly delayed in the presence of N-acetylcysteine or β-carotene. The present results suggest that certain photosensitizing compounds may affect the color and stability of MTT formazan, which should be carefully considered when conducting the MTT assay.

목차

Abstract
 서론
 재료 및 방법
  실험 재료
  세포배양 및 MTT formazan의 추출
  ZnPP에 의한 세포독성 평가와 발색도 변화 측정
  MTT formazan의 추출
  MTT formazan의 발색도에 미치는 각종 영향요인 분석
  결과 분석 및 통계처리
 결과 및 고찰
  ZnPP에 의한 MTT formazan 발색도의 변화
  ZnPP에 의한 MTT formazan 탈색에 미치는 영향요인
  ZnPP에 의한 formazan dye의 탈색에 미치는 항산화제의 영향
 요약
 감사의 글
 문헌

키워드

저자

• 박경아 [ Kyung A Park | 서울여자대학교 자연과학대학 식품공학과 ]
• 최현아 [ Hyun A Choi | 서울여자대학교 자연과학대학 식품공학과 ]
• 김미리 [ Mi-Ri Kim | 서울여자대학교 자연과학대학 식품공학과 ]
• 최유미 [ Yoo Mi Choi | 서울여자대학교 자연과학대학 식품공학과 ]
• 김현정 [ Hyun Jung Kim | 서울여자대학교 자연과학대학 식품공학과 ]
• 홍정일 [ Jungil Hong | 서울여자대학교 자연과학대학 식품공학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국식품과학회지 [Korean Journal of Food Science and Technology]

• 간기

  격월간

• pISSN

  0367-6293

• 수록기간

  1969~2011

• 십진분류

  KDC 574 DDC 664