Earticle

초록

영어

Human cytotoxic T-lymphocyte antigen 4- immunoglobulin (hCTLA4Ig), an immunosuppressive agent, was expressed in transgenic rice cells using RAmy3D promoter and RAmy1A signal peptide for the inducible production and secretion into culture media by sugar depletion. In this study, sodium butyrate was used as a small molecular enhancer (SME) to enhance the production of hCTLA4Ig in transgenic rice cell suspension cultures. When 1 mM sodium butyrate was added in sugar-free media, relative viability was not reduced, while the productivity was improved 1.3-fold. In addition, by supplementing 87 mM sodium pyruvate as an alternative energy source during the production phase, death rate of the cells was decreased. When sodium pyruvate was not added, most cells became dead at day 6. However, by adding sodium pyruvate, 18% of viability can be maintained until day 10 and the production of hCTLA4Ig was enhanced 1.4-fold. When the combination of sodium pyruvate and sodium butyrate at optimum concentrations was added, the highest viability and hCTLA4Ig production could be obtained. The highest level of hCTLA4Ig reached up to 35 mg/L at day 10.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 세포주 및 세포배양
  2.2. 세포량 측정
  2.3. Cell viability 측정
  2.4. Protease 활성 측정
  2.5. 총단백질 정량
  2.6. hCTLA4Ig 정량
  2.7. Total genomic DNA 분석
 3. 결과 및 고찰
  3.1. Sodium butyrate 첨가가 미치는 영향
  3.2. 에너지원 첨가의 영향
  3.3. Sodium butyrate와 sodium pyruvate 동시 첨가에 따른 영향
 4. 결론
 감사
 References

키워드

저자

• 유미희 [ Mi-Hee Yoo | 인하대학교 공과대학 생물공학과 ]
• 김수진 [ Soo-Jin Kim | 인하대학교 공과대학 생물공학과 ]
• 권준영 [ Jun-Young Kwon | 인하대학교 공과대학 생물공학과 ]
• 남형진 [ Hyung-Jin Nam | 인하대학교 공과대학 생물공학과 ]
• 김동일 [ Dong-Il Kim | 인하대학교 공과대학 생물공학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576