Earticle

초록

영어

As a number of archaea are ubiquitously found in non-extreme habitats, elucidation of their functional roles becomes currently an emerging issue. However, most of them are unable to grow in pure culture and so it remains to be established. In order to find genes lacking in the genome of an ammonia-oxidizing archaeon (AOA), we here report on the comparative analyses of an AOA genome with those of experimentally or theoretically established minimal genomes for independent growth. We assessed the genes lacking in AOA using logic of clusters of orthologous groups (COG), remote homology, consensus sequence weight matrix, function-based motif or domain, and then further excluded genes encoding hypothetical orarchaea-specific proteins. The results of these combination analyses revealed 19 candidate genes lacking in the genome of an AOA. Thus, our results provide a possibility of inducing independent growth of AOA when supplemented with product (s) of the lacking gene (s), and also give a chance for finding new proteins with novel sequence or structure space even if the predicted lacking-genes will be found using another algorithms or biochemical studies.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 해양 고세균과 비교에 사용된 박테리아 유전체 서열
  2.2. 유전체 비교 분석 과정
  2.3. 결손 추정 유전자가 관여하는 대사 경로 분석과 대사물 첨가에 의한 세포 성장 분석
 3. 결과 및 고찰
  3.1. 결손 유전자 탐색에 이용한 N. martimus SCM1과 AR enrichment culture 와의 연관성
  3.2. 해양 고세균에서 결손된 것으로 보이는 필수 유전자 분석도구와 비교 대상 유전체
  3.3. 해양 고세균 N. martimus SCM1 유전체에서 결손 유전자탐색
  3.4. 결손 추정 유전자와 관련 있는 대사회로와 대사물
 4. 결론
 감사
 References

키워드

저자

• 한상수 [ Sang-Soo Han | 전남대학교 생물학과 ]
• 이진영 [ Jin-Young Lee | 전남대학교 생물학과 ]
• 이성근 [ Sung-Keun Rhee | 충북대학교 미생물학과 ]
• 김근중 [ Geun-Joong Kim | 전남대학교 생물학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576