Earticle

초록

영어

Despite of the absence of hyperacute rejection and acute humoral xenograft rejection, the organ graft of the a1,3-galactosyltransferase (GalT) gene knockouted (KO) and complement regulatory protein (CRP) expressing pig into a nonhuman primate is rejected by development of a thrombotic microangiopathy and/or a consumptive coagulopathy. Thus further introduction of genes to overcome the coagulation incompatibilities between pig and primate under GalT KO/CRP genetic background has been strongly suggested. CD73 (ecto-5'-nucelotidase) is an enzyme attached via a glycosyl phosphoinositol anchor to the extracellular membrane of endothelial cells, which catalyses the hydrolysis of adenosine triphosphate to adenosine. Loss of activity of CD73 results in activation and aggregation of platelets by a reduced capacity to convert nucleotides to adenosine. In previous study, we reported generation of GalT KO fibroblasts concurrently expressing membrane cofactor protein and produced cloned pigs by nuclear transfer of the fibroblast cells (1). In this study, we constructed a vector for expression of human CD73 under control of promoter of pig Icam2 gene expressed specifically at endothelial cells. This vector was introduced into porcine fibroblasts using the nucleofection technology, by which we had forty three fibroblasts clones carrying pIcam2- CD73 vector. Somatic cell nuclear transfer resulted in generation of two transgenic piglets survived.

키워드

저자

• Keon Bong Oh [ Animal Biotechnology Division National Institute of Animal Science, RDA ]
• Bella Kim [ Animal Biotechnology Division National Institute of Animal Science, RDA, School of Life Science and Biotechnology, Kyungpook National University ]
• Nayoung Ko [ Animal Biotechnology Division National Institute of Animal Science, RDA, College of animal life science, Kangwon National University ]
• Dae-Jin Kwon [ Animal Biotechnology Division National Institute of Animal Science, RDA ]
• Dong-Hoon Kim [ Animal Biotechnology Division National Institute of Animal Science, RDA ]
• Gi-Sun Im [ Animal Biotechnology Division National Institute of Animal Science, RDA ]
• Jin-Ki Park [ Animal Biotechnology Division National Institute of Animal Science, RDA ]
• Seongsoo Hwang [ Animal Biotechnology Division National Institute of Animal Science, RDA ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636