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Change Activity and the Inhibitory Factor of Plasminogen Activator in Porcine Uterus Tissue

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 11th International Symposium on Developmental Biotechnology (2011.10)바로가기
  • 페이지
    pp.86-86
  • 저자
    Yong-Seung Lee, Ha-Na Gu, Hee-Tae Cheong, Boo-Keun Yang, Seung-Hwan Lee, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A156608

원문정보

초록

영어
The present study was performed to identify the role of plasminogen activator (PA) and the location of PA expression in porcine uterus tissues during the estrous cycle. Porcine uterus tissues were obtained from ovary in pre-ovulatory (Pre-Ov), post-ovulatory stage (Post-Ov) and early to mid-luteal stage (Early-mid L). The uterus tissue was immediately fixed by PBS with 10% formalin. There were fixed porcine uterus tissue for 24 hours at room temperature and porcine uterus tissue dehydrate for 12 hour in sucrose solution. For immunohistochemical staining, porcine uterus tissues were cut to 4 μm by micro frozen section microtome. The nucleus and cytoplasm of porcine uterus tissues were stained by Hematoxin and Eosin. Porcine uterus tissues were evaluated by Immunofluorescence using anti-tissue type PA (tPA) and urokinase type PA (uPA). The location of PA expression was identified by observing the PA fluorescence using fluorescent microscope and optical telescopes. As a results, when Pre-Ov and Post-Ov were identified endometrial blood vessel in an inner layer that were observed tPA and uPA. Especially, expression of PA was observed around secretory gland. But the expression of PA were not confirm in Early-mid L. Also, The expression of PA were higher in Post-Ov than Early-mid L. In conclusion, during the estrous cycle, the expression of PA were increased from Pre-Ov to Post-Ov and was decreased from Post-Ov to Early-mid L.

키워드

Porcine uterus tissues Estrous cycle Plasminogen activator

저자

  • Yong-Seung Lee [ College of Animal Life Sciences ]
  • Ha-Na Gu [ College of Veterinary Medicine Kangwon National University ]
  • Hee-Tae Cheong [ College of Veterinary Medicine Kangwon National University ]
  • Boo-Keun Yang [ College of Animal Life Sciences ]
  • Seung-Hwan Lee [ National Institute of Animal Science, Hanwoo Ex ]
  • Choon-Keun Park [ College of Animal Life Sciences ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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