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Synergic Effects of MEM Vitamins and Beta-mercaptoethanol in Maturation Medium on ROS of Oocytes and Embryos, and Apoptosis of Blastocysts in Porcine

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 11th International Symposium on Developmental Biotechnology (2011.10)바로가기
  • 페이지
    pp.65-66
  • 저자
    Sang-hyoun Park, Jong-hoon Kim, Jung-kee kwon, Il-jeoung Yu
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A156589

원문정보

초록

영어
In this study, synergic effects of MEM vitamins (MEMv) and beta-mercaptoethanol (bME) supplemented to porcine maturation medium on reactive oxygen species (ROS) of oocytes and embryos, and apoptosis of blastocysts were determined. Cumulus-oocyte- complexes (COCs) were allocated into four treatment groups: 0.05X MEMv, 25 uM bME, 0.05X MEMv + 25 uM bME or a group without MEMv + bME. In experiment 1, COCs were cultured in four respective treatment groups based on NCSU-23 medium for 44 h at 39℃ in a 5% CO2 atmosphere. We evaluated ROS of oocytes. In experiment 2, COCs were cultured in four respective treatment groups and then were fertilized in vitro (IVF) or activated by chemical or electrical method. We determined ROS of early stage embryos (2 cell-4 cells) and apoptosis of blastocysts. DCHFDA for ROS level and TdT-mediated dUTP nick end labelling (TUNEL) for apoptosis were used. As a result, ROS level of oocytes was not significant difference among experimental groups. In early stage embryos produced by IVF, MEMv + bME group showed significantly lower ROS level than that of control group (p<0.05). Level of apoptosis in blastocysts of the MEMv + bME group was significantly lower than that of the control group (p<0.05). In early stage embryos produced by chemical activation, ROS level of MEMv + bME group was significantly lower than that of bME group (p<0.05) without significant difference with those of control and MEMv group. Level of apoptosis in blastocysts in the MEMv + bME group was significantly lower than that of the control group (p<0.05). In early stage embryos produced by electrical activation, ROS level of MEMv + bME group was significantly lower than that of control (p<0.05). However, apoptosis level of blastocyst was not significant difference among experimental groups. In conclusion, the present study indicates that the addition of MEM vitamins and betamercaptoethanol during in vitro maturation is able to alleviate the production of ROS and apoptosis.

키워드

MEM vitamins Beta-mercaptoethanol ROS Apoptosis Porcine oocytes Porcine embryos

저자

  • Sang-hyoun Park [ College of Veterinary Medicine ＆ Bio-safety Research Institute, Chonbuk National University ]
  • Jong-hoon Kim [ College of Veterinary Medicine ＆ Bio-safety Research Institute, Chonbuk National University ]
  • Jung-kee kwon [ College of Veterinary Medicine ＆ Bio-safety Research Institute, Chonbuk National University ]
  • Il-jeoung Yu [ College of Veterinary Medicine ＆ Bio-safety Research Institute, Chonbuk National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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