Earticle

초록

영어

Successful early embryogenesis of somatic cell nuclear transfer (SCNT) embryos is very important to produce cloned animals. However, poor preimplantation development of SCNT embryos has been a major obstacle to the generation of cloned animals due to a lack of understanding of developmental events and underlying mechanism(s). In the current study, we show that production of SCNT embryos with high developmental competence is dependent on the fusion method. Electrofusion causes spontaneous egg activation, accompanied by an increase in intracellular Ca2+ and improper nuclear remodeling, whereas Sendai virus (SV)-mediated fusion greatly reduces these events. In addition, SV-SCNT increased the blastocyst development rate and trophectoderm cell number compared to electrofusion-mediated SCNT (E-SCNT). In particular, expression of ER stress-associated genes and blastomere apoptosis were significantly increased in E-SCNT embryos, which could be alleviated by inhibition of ER stress or by using the SV-mediated fusion method. Taken together, these results strongly suggest that SV is a useful fusion material for improvement of preimplantation development of SCNT embryos through reduction of ER stress-associated apoptosis.

키워드

저자

• Bong-Seok Song [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Ji-Su Kim [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Bo-Woong Sim [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Seung-Bin Yoon [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Young-Hyun Kim [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Department of Functional Genomics, University of Science and Technology (UST) ]
• Jae-Jin Cha [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Seon-A Choi [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Hyun-Ki Min [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Sun-Uk Kim [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Department of Functional Genomics, University of Science and Technology (UST) ]
• Kyu-Tae Chang [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Department of Functional Genomics, University of Science and Technology (UST) ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636