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Ovum Pick up and In Vitro Embryo Production in Hanwoo

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 11th International Symposium on Developmental Biotechnology (2011.10)바로가기
  • 페이지
    pp.35-35
  • 저자
    Youn Bae Park, Keun Jung Kim, Ji Hye Lee, Kang Sun Park, Eun Young Kim, Maria Kim, Kil woo Han, Xiaoxia Li, Jeong Yu, Dong Soo Lee, Min Kyu Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A156560

원문정보

초록

영어
The objective of this study was to evaluate in vitro production of bovine embryos in Hanwoo. Oocytes were collected by ovum pick up (OPU) from ovaries of genetically high-value Hanwoo or by needle puncture from ovaries of slaughtered cattle. OPU was done every 3 4 days duing experimental period and collected oocytes were fertilized in vitro in both OPU and needle puncture groups. First, We compared the in vitro maturation rate in two groups (Experiment 1). 545 oocytes were recoverd from 4 females by 32 trials of OPU and then 433 oocytes were shown MⅡ stage after in vitro maturation (79.4%). In case of needle puncture group, 1905 oocytes were collected and then 1420 oocytes were matured to MⅡ stage during in vitro culture(74.5%). Second, we compared the developmental rate to blastocyst in two groups (Experiment 2). 1420 oocyte by needle puncture were fertilized with frozen-thawing semen; the cleavage rate 24 48 h after in vitro fertilization (IVF) was 88.6% and blastocyst development rate was 20.5% in needle puncture group. Even though there is lower cleavage rate after IVF in OPU group (84.8%), blastocyst development rate was higher compared with needle puncture group (26.4%). In conclusion, Blastocyst developmental rate could be increased by OPU than classical method of needle puncture. Improvement of bio-technique in collecting oocytes could be applied to understand the reproductive physiology in cattle, expecially Hanwoo. Therefore, further investigation should be done to clarify the efficiency and advantage of OPU involved in reproduction in animals and human being. This research was suppoted by Imsil-gun agricultural technology service center.

키워드

Ovum pick up In vitro production Hanwoo

저자

  • Youn Bae Park [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Keun Jung Kim [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Ji Hye Lee [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Kang Sun Park [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Eun Young Kim [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Maria Kim [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Kil woo Han [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Xiaoxia Li [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Jeong Yu [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Dong Soo Lee [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]
  • Min Kyu Kim [ Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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