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Induction of Pluripotency and Differentiation

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 11th International Symposium on Developmental Biotechnology (2011.10)바로가기
  • 페이지
    pp.19-19
  • 저자
    Jeong Tae Do
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A156548

원문정보

초록

영어
Somatic cells achieve a pluripotent state by pluripotential reprogramming. During pluripotential reprogramming, somatic cells re-established various features of pluripotent cells, such as the expression of pluripotency markers, inactivation of tissue-specific gene expression, developmental potential to contribute to all three germ layers, and an undifferentiated epigenetic state. Induced pluripotent stem (iPS) cells undergo unlimited self-renewal and have differentiation potential into various types of cells like embryonic stem cells. These iPS cells are potentially a valuable source of immune matched pluripotent stem cells that can be differentiated and used for tissue replacement therapies. Recent technical advance in direct reprogramming of somatic cells lead to a safe, viral- free iPS cell generation. Here we develop new techniques to generate iPS cells. Using titanium oxide (TiO2) nanotubes. we could successfully transfer reprogramming proteins (Oct4, Sox2, Klf4, Nanog and c-Myc) into somatic cells. After two weeks of treatment of protein conjugated nanotubes, somatic cells adopted an ES cell-like morphology and activated Oct4-GFP, which is pluripotency marker, indicating that nanotubes can be used for protein delivery carriers, which induce cellular reprogramming. Next, we induced differentiation of iPS cells into neural stem cells (NSCs) and compared with mouse embryonic stem (ES) cell-derived NSCs. NSCs from ES and iPS cells were morphologically indistinguishable from NSCs from brain tissue and rapidly propagated in the presence EGF and bFGF, and stained positive for NSCs markers Nestin and Sox2. Moreover, these NSCs have capacity of differentiation into multiple cell lineages, such as neurons, astrocytes, and oligodendrocytes. Induction of pluripotency and directed differentiation of iPS cells into a specialized cell type hold considerable promise for regenerative medicine as well as basic research.

저자

  • Jeong Tae Do [ Laboratory of Stem Cell and Developmental Biology, Department of Biomedical Science, CHA University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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