The aim of this study was to evaluate the effect of α-tocopherol on blastocysts development and subsequent cryosurvival of the vitrification. The α-tocopherol(0, 100, 200, 400 μM) was added in to culture medium for the bovine embryos. The blasocysts from the α-tocopherol and untreated control groups were then frozen-thawed, and their cryosurvival was assessed by in vitro culture for 48 h. There were no differences in the overall cleavage rate(56.14±4.66, 58.18±4.70, 62.97±6.86 and 51.17±7.28) among four treatment groups. However, in blastocyst development and total cell number were significantly higher in α-tocopherol 200 μM(38.60±7.12; 106.33±3.50) to culture medium than other treatment groups(29.30±5.24, 31.60±7.12 and 26.37±4.18; 101.36±5.12, 97.27±2.87, and 91.23±7.52 respectively). Before and after vitrification, the total cell number and blastocyst development of embryo were significantly higher in July to August than September to October. In conclusion, addition of α-tocopherol 200 μM to in vitro bovine embryo culture medium was beneficial for improving embryo quality by decreasing the embryo damage blsstocysts cell number and improving the tolerance of the embryos to cryopreservation.
목차
ABSTRACT 서론 재료 및 방법 난포란의 체외성숙과 수정 수정란의 체외 배양 α-Tocopherol 첨가 배양 초자화 동결 및 융해 수정란의 세포수 조사 통계 분석 결과 및 고찰 α-Tocopherol 첨가에 따른 분할율 α-Tocopherol 첨가에 따른 배반포 발달율 α-Tocopherol 첨가에 따른 총 세포수 α-Tocopherol 첨가에 따른 초자화 동결 · 융해 후 총세포수 적요 인용문헌
키워드
BovineEmbryosIn vitroIn vivoVitrification
저자
정진우 [ Jin-Woo Jung | 농협중앙회 한우개량사업소 ]
손준규 [ Jun-Kyu Son | 농촌진흥청 국립축산과학원 ]
최창용 [ Changyong Choe | 농촌진흥청 국립축산과학원 ]
서상원 [ Sangwon Suh | 농촌진흥청 국립축산과학원 ]
최진석 [ Jin-Seok Choi | 농촌진흥청 국립축산과학원 ]
조인철 [ In-Cheol Cho | 농촌진흥청 국립축산과학원 ]
강태영 [ Tae-Young Kang | 제주대학교 수의학과 ]
한상현 [ Sang-Hyun Han | 농촌진흥청 국립축산과학원, 제주대학교 수의학과 ]
강민수 [ Min-Soo Kang | 제주대학교 수의학과 ]
김덕임 [ Duck-Im Kim | 농협중앙회 한우개량사업소 ]
김현종 [ Hyun-Jong Kim | 농촌진흥청 국립축산과학원 ]
조상래 [ Sang-Rae Cho | 농촌진흥청 국립축산과학원 ]
Corresponding author