Earticle

초록

영어

The purpose of this study was to improve of frozen-thawed sperm using magnetized water in Korean native cattle. Before cryopreservation, without egg-yolk Triladyl® solution was flowed though magnet [0, 2000, 4000 and 6000 Gauss(G)] for 5 min. The freezing of dilluted semen added with Triladyl containing 20% egg-yolk. Analysis of frozen-thawed sperm was estimated viability with SYBR14/PI double stain, membrane intact with hypoosmotic swelling test (HOST), acrosome reaction with FITC-PNA, mitochondria membrane function with Rhodamin 123 by flow- cytometry. Sperm viability was significantly higher in 4000G group than other groups (p<0.05). However, the Hypoosmotic Swelling Test(HOST) was significantly higher in fresh, 4000 and 6000G than 0 and 2000G (p<0.05). In addition, mitochondria membrane damage and acrosome damage were significantly lower in 6000G group than other groups (p<0.05). in conclusion we suggest that magnetized water could be improve ability of sperm on cryopreservation in Korean native cattle.

목차

ABSTRACT
 서론
 재료 및 방법
  정액 채취
  정액 동결
  동결정액 융해 및 성상검사
  생존율 검사(SYBR-14/PI Staining)
  정자원형질막 기능 검사(Hypoosmotic Swelling Test)
  미토콘드리아 검사(Rhodamin123/PI Staining)
  첨체검사(FITC-PNA/PI Staining)
  통계처리(Statistical Analysis)
 결과
  자기활성된 동결액 처리 후 생존율 평가
  정자의 원형질막 검사(Hypoosmotic Swelling Test)
  정자의 미토콘드리아 검사
  자기활성된 동결액 처리 후 정자의 첨체 손상 검사
 고찰
 인용문헌

키워드

저자

• 서기범 [ Gi-Beom Seo | 강원대학교 동물생명과학대학 ]
• 이용승 [ Yong-Seung Lee | 강원대학교 동물생명과학대학 ]
• 이경진 [ Kyung-Jin Lee | 강원대학교 동물생명과학대학 ]
• 유한준 [ Han-Jun Yu | 강원대학교 동물생명과학대학 ]
• 정희태 [ Hee-Tae Cheong | 강원대학교 수의학과 ]
• 양부근 [ Boo-Keun Yang | 강원대학교 동물생명과학대학 ]
• 이승환 [ Seung-Hwan Lee | 국립축산과학원 한우시험장 ]
• 이진우 [ Jin-Woo Lee | ROHASTECH ]
• 박춘근 [ Choon-Keun Park | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636