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Involvement of Ski Protein Expression in Luteinization in Rat Granulosa Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 35 No 3 (2011.09)바로가기
  • 페이지
    pp.355-361
  • 저자
    Hyun Kim, Takashi Matsuwaki, Keitaro Yamanouchi, Masugi Nishihara, Sungwoo Kim, Yeoung-Gyu Ko, Boh-Suk Yang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A155992

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원문정보

초록

영어
Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to locate Ski protein in the rat ovary during luteinization to predict the possible role of Ski. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rat, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum (CL). Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally.

목차

INTRODUCTION
 MATERIALS AND METHODS
  Animals
  Hormone Assay
  Immunohistochemistry and TUNEL Staining
  qPCR (Real Time PCR)
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Arkadia Granulosa cells Luteinization Ski

저자

  • Hyun Kim [ Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Animal Genetic Resources Station, National Institute of Animal Science, RDA ] Corresponding author
  • Takashi Matsuwaki [ Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo ]
  • Keitaro Yamanouchi [ Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo ]
  • Masugi Nishihara [ Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo ]
  • Sungwoo Kim [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Yeoung-Gyu Ko [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Boh-Suk Yang [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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