Earticle

초록

영어

Fibroblasts of large animals are easy to isolate and to maintain in vitro culture. Thus, these cells are extensively applied to donor cell for somatic cell nuclear transfer, and to substrate cells to generate induced pluripotent stem cells after transfection of required genes to be essentially required for direct reprogramming. However, limited mitotic activity of fibroblasts to differentiate along a terminal lineage becomes restrictive for their versatile application. Recently, commercial culture medium and systems developed for primary cells are provided by manufactures. In this study, we examined whether one of the systems developed for primary fibroblasts of human are effective on porcine ear skin fibroblasts. To this end, we performed proliferation assay after five days culture in vitro of porcine fibroblasts in medium DMEM, which is generally used for fibroblasts culture, and medium M106 for human dermal fibroblasts, supplemented with various concentrations of FBS and LSGS contained mainly growth factors, respectively. Consequence was that presence of 15% FBS and 0.1 X concentrations of LSGS in DMEM showed most active proliferation of porcine fibroblasts.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Isolation of Porcine Fibroblasts
  Porcine Fibroblasts Culture for Proliferation Assay
  WST-1 Cell Proliferation Assay
  Coomassie Blue Staining of Fibroblast
 RESULTS AND DISCUSSION
 REFERENCES

키워드

저자

• Bella Kim [ Animal Biotechnology Division, National Institute of Animal Science, RDA, School of Life Science and Biotechnology, Kyungpook National University ]
• Nayoung Ko [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Department of Animal Biotechnology, Kangwon National University ]
• Seong-soo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Gi-Sun Im [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Dong-Hoon Kim [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Jin-Ki Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Zae Young Ryoo [ School of Life Science and Biotechnology, Kyungpook National University ]
• Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, RDA ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636