Earticle

초록

영어

This study was undertaken to evaluate the relationship between in vitro maturation and plasminogen activators (PAs) activity on porcine cumulus-oocytes complexes (COCs) exposed to oxidative stress. When COCs were cultured in maturation medium with hydrogen peroxide (H2O2), the proportion of the germinal vesicle breakdown (GVBD) and oocytes maturation were decreased with addition of H2O2, and were significantly (p<0.05) lower in medium with 0.1 mM H2O2 than control group. Also, the rate of degenerated oocytes was increased in as H2O2 concentration increased.
When COCs were cultured for 48 h, three plasminogen-dependent lytic bands were observed: tissue-type PA (tPA); urokinase-type PA (uPA); and tPA-PA inhibitor (tPA-PAI). PA activity was quantified using SDS-PAGE and zymography.
When H2O2 concentration was increased, tPA and tPA-PAI activities also increased in porcine oocytes cultured for 48 h, but not uPA. In other experiment, embryos were divided into three groups and cultured in (1) control medium, (2) control medium with 1.0 mM H2O2 and (3) control medium with 1.0 mM H2O2 along with catalase in concentrations of 0.01, 0.1, and 1.0 mg/ml, respectively. H2O2 decreased the rate of GVBD and maturation in porcine COCs but catalase revealed protective activity against oxidative stress caused by H2O2. In this experiment, tPA and tPA-PAI activities were higher in media with 1.0 mM H2O2 alone. Increasing concentration of catalase decreased tPA and tPA-PAI activities in porcine oocytes. These results indicate that the exposure of porcine follicular oocytes to ROS inhibits oocytes maturation to metaphase-II stage and increase the oocytes degeneration. Also, we speculated that increased ROS level may trigger tPA and tPA-PAI activities in porcine oocytes matured in vitro.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Culture Media
  Preparation of Oocytes
  Experimental Design
  SDS-PAGE and Zymography
  Statistics
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자

• Soo Jin Sa [ Swine Science Division, National Institute of Animal Science, RDA ]
• Chun Keun Park [ College of Animal Life Science, Kangwon National University ]
• Hee Tae Cheong [ Veterinary Medicine, Kangwon National University ]
• Jung Ho Son [ Noah Biotech. Inc. ]
• Myung Jick Kim [ Swine Science Division, National Institute of Animal Science, RDA ]
• Kyu Ho Cho [ Swine Science Division, National Institute of Animal Science, RDA ]
• Du Wan Kim [ Swine Science Division, National Institute of Animal Science, RDA ]
• Kyoung Min So [ Swine Science Division, National Institute of Animal Science, RDA ]
• In Cheul Kim [ Swine Science Division, National Institute of Animal Science, RDA ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636