Earticle

초록

영어

After Ganoderma lucidum was cultured in mushroom complete medium (MCM) supplemented with ginseng extract (GE), crude polysaccharide (GL-GE-CP) was fractionated from mycelium. Among GL-GE-CP from mycelium in MCM supplemented with 5, 10, and 15% GE (v/v ratio of MCM to GE), GL-GE- 15-CP (15% GE) most significantly enhanced macrophage stimulation and intestinal immune system modulating activity compared with GL-CP in MCM without GE. When GL-GE-15-CP was further fractionated on DEAE-Sepharose CL-6B, GL-GE-15-CP-II displayed more potent activity than subfractions from GL-CP on macrophage stimulation, interleukin-12 production, and intestinal immune system modulation (1.75-, 5.68-, and 1.76-fold, respectively). Anti-metastasis effect against colon 26-M3.1 carcinoma cells was also enhanced by GL-GE-15-CP-II (72.8% inhibition). In addition, GL-GE- 15-CP-II contained neutral sugar (83.00%) and uronic acid (9.11%), and consisted of Ara, Man, Gal and Glc (molar ratio of 0.39:0.50:0.75:1.00). Furthermore, GE supplementation helped to enhance the immunomodulation in G. lucidum, and it is assumed that neutral polysaccharides play an important role.

목차

Abstract
 서론
 재료 및 방법
  영지버섯 균주 및 수삼추출물 조제
  수삼추출물 첨가 혼합 액체배지에서 배양된 영지버섯 균사체
  수삼추출물-영지버섯 균사체로부터 면역활성 조다당의 조제
  수삼추출물-영지버섯 균사체 조다당으로부터 면역활성 다당류의 분획
  조다당으로부터 분획된 면역활성 다당획분의 구성분 및 구성당 분석
  실험동물과 시약
  마크로파지 활성 및 IL-12 생산능
  Peyer’s patch를 경유한 장관면역 활성
  종양전이 억제활성
  통계처리
 결과 및 고찰
  수삼추출물-영지버섯 균사체의 열수추출물 및 조다당획분의 면역활성
  조다당획분의 DEAE-Sepharose CL-6B 분획 및 면역활성
  수삼추출물-영지버섯 균사체로부터 분리된 면역활성 다당획분의 특성
 요약
 감사의 글
 문헌

키워드

저자

• 김훈 [ Hoon Kim | 충주대학교 식품공학과, (주)코시스바이오 기업부설연구소 ]
• 정재현 [ Jae-Hyun Jeong | 충주대학교 식품공학과 ]
• 정헌상 [ Heon-Sang Jeong | 충북대학교 식품공학과 ]
• 황종현 [ Jong-Hyun Hwang | 충주대학교 식품영양학과 ]
• 유광원 [ Kwang-Won Yu | 충주대학교 식품영양학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국식품과학회지 [Korean Journal of Food Science and Technology]

• 간기

  격월간

• pISSN

  0367-6293

• 수록기간

  1969~2011

• 십진분류

  KDC 574 DDC 664