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The Development of a Column-Switching High-Performance Liquid Chromatograph System for Chiral Separation of Mouse Urinary D, L-Lactate
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- 발행기관
- 보안공학연구지원센터(IJBSBT) 바로가기
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- 간행물
- International Journal of Bio-Science and Bio-Technology SCOPUS 바로가기
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- 통권
- vol.2 no.1 (2010.03)바로가기
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- 페이지
- pp.31-38
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A147451
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Two high-performance liquid chromatograph (HPLC) systems with a Rheodyne Model 7000 switching valve for separation of D- and L-lactate in biological samples has been reported. However, since the switching valve was rotated manually, so that only 10 samples could be determined within one day. For better efficiency, a circuit for rotating the switching valve automatically, which is including mechanical units of motion, two timers, and electronic control units was designed. Total (D+L)-lactate fluorescent derivatives was separated from others by a TSKgel ODS-80Ts column, when the peak of lactate derivatives appeared in the chromatogram on the ODS column, the valve position was changed by switching and then the isolated lactate derivatives was introduced into the chiral column automatically. Then the D- and L- enantiomers were separated by the chiral column. The utility of the analyzer system was tested for the determination of chiral separation of urinary D, L-lactate in the mouse. It takes about 90 min to analyze one urine sample for a circulation. Before automation, we must turn the switching valve manually when the signal shows up, so that there must be someone always standby and only about 10 samples could be analyzed within one day. With the designed automatic column-switching HPLC system, the switching valve can be automatically turned at the setting time repeatedly and over 15 samples could be determined within one day. The automatic system makes the analysis of D-lactate be timesaving, convenient and reproducible.
목차
Abstract
1. Introduction
2. Experimental
3. Application
4. Conclusion
References
키워드
Column-switching HPLC; Switching valve; Automation; Chiral separation; D-lactate.저자
간행물 정보
발행기관
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- 발행기관명
- 보안공학연구지원센터(IJBSBT) [Science & Engineering Research Support Center, Republic of Korea(IJBSBT)]
- 설립연도
- 2006
- 분야
- 공학>컴퓨터학
- 소개
- 1. 보안공학에 대한 각종 조사 및 연구 2. 보안공학에 대한 응용기술 연구 및 발표 3. 보안공학에 관한 각종 학술 발표회 및 전시회 개최 4. 보안공학 기술의 상호 협조 및 정보교환 5. 보안공학에 관한 표준화 사업 및 규격의 제정 6. 보안공학에 관한 산학연 협동의 증진 7. 국제적 학술 교류 및 기술 협력 8. 보안공학에 관한 논문지 발간 9. 기타 본 회 목적 달성에 필요한 사업
간행물
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- 간행물명
- International Journal of Bio-Science and Bio-Technology
- 간기
- 격월간
- pISSN
- 2233-7849
- 수록기간
- 2009~2016
- 등재여부
- SCOPUS
- 십진분류
- KDC 505 DDC 605
이 권호 내 다른 논문 / International Journal of Bio-Science and Bio-Technology vol.2 no.1
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