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Generation of Reactive Oxygen Species in Porcine Parthenogenetic Embryos

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 35 No 2 (2011.06)바로가기
  • 페이지
    pp.191-195
  • 저자
    In-Sun Hwang, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A145793

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초록

영어
The present study was conducted to examine the reactive oxygen species (ROS) generation levels in porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by the combination of electric stimulus and 6‐ DMAP before in vitro culture. Porcine oocytes and parthenogenetic embryos were stained in 10 μM dichlorohydrofluorescein diacetate (DCF) or 10 μM hydroxyphenyl fluorescein (HPF) dye each for 30 min at 39℃. The fluorescent emissions from the samples were recoded as JPEG file and the intensity of fluorescence in oocytes and embryos were analyzed. H2O2 and ˙OH radical levels of porcine oocytes were reduced immediately after electric stimulation. However, H2O2 and ˙OH radical levels of parthenogenetic embryos were increased with time elapsed after electric stimulation from 0 h to 3 h and after DMAP culture. During in vitro culture, H2O2 and ˙OH radical levels were gradually increased from the one‐cell stage to the two‐ and four‐cell stages. The result of the present study suggests that the ROS was not increased by electric pulse in porcine embryos. Rather than it seems to be associated with the stage of development and the culture condition.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  In Vitro Maturation (IVM) of Oocytes
  Parthenogenesis and In Vitro Culture
  Analysis of ROS Products
  Experimental Designs
  Statistical Analysis
 RESULTS
  ROS Levels at Activation Procedures
  ROS Levels by the Time Elapsed after Electric Stimulation
  ROS Levels of Parthenogenetic Embryos during In Vitro Culture
 DISCUSSION
 REFERENCES

키워드

Reactive oxygen species H2O2 .OH radical Parthenogenesis Porcine

저자

  • In-Sun Hwang [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University ]
  • Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University ]
  • Boo-Keun Yang [ College of Animal Life Sciences, Kangwon National University ]
  • Hee-Tae Cheong [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 35 No 2

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