Earticle

초록

영어

To evaluate the effect of spermatozoa culture on glycosidase activity of frozen-thawed spermatozoa in human, the spermatozoa were treated experimentally and assayed for activities of α-L-fucosidase, α-D-mannosidase, β-D-galactosidase and N-acetyl-β-D-glucosaminidase (β-GlcNAc’ase). The β-GlcNAc’ase activity was at least two-folds higher than other glycosidases regardless of spermatozoa incubation (p<0.05). The spermatozoa motility was decreased with incubation periods, but no effects by different glycosidases on the changes of spermatozoa motility during the various periods of incubation. In all glycosidases, the spermatozoa-zona binding rates in spermatozoa without incubation were higher than in spermatozoa incubated for 2 h (p<0.05). β-GlcNAc’ase is present mainly in the plasma membrane of spermatozoa frozen-thawed in human. It was also shown that the glycosidase activity was increased in all glycosidases in spite of lower sperm-zona binding by spermatozoa incubation.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Semen Collection and Preparation
  Treatment of Spermatozoa for Glycosidase Analyses
  Measurement of Glycosidase Activity
  Chlortetracycline (CTC) Assessment of Spermatozoa
  Spermatozoa Zona-Binding Test
  Experimental Design
 RESULTS
 DISCUSSION
 ACKNOWLEDGEMENTS
 REFERENCES

키워드

저자

• Chae-Sik Lee [ College of Animal Life Sciences, Kangwon National University, Saewha Women’s Clinic ]
• Sang-Chan Lee [ Saewha Women’s Clinic ]
• Ji-Eun Lee [ College of Animal Life Sciences, Kangwon National University ]
• Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University ]
• Boo-Keun Yang [ College of Animal Life Sciences, Kangwon National University ]
• Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636