Earticle

초록

영어

Arthritis is a common disease in aged people, and is clinically divided into rheumatoid arthritis (RA) and osteoarthritis (OA). Although common symptoms such as pain are present, the underlying pathological mechanisms are slightly different. Therefore, the objectives of the present study were to compare joint damage induced by RA and OA by analyzing the major morphological and molecular differences, and to propose a suitable therapeutic intervention based on the pathophysiological conditions of bones and joints. For the RA animal model, 8-week-old DBA1/J mice were immunized with bovine type II collagen emulsified in complete Freund’s adjuvant (CFA). Normal C57BL/6 mice (over 2 years of age) were used for OA. The clinical arthritis score was calculated using a subjective scoring system, and paw thicknesses were measured using calipers. The serum TNFα level was analyzed using an ELISA kit. Micro- CT was used to identify pathological characteristics and morphological changes. In collagen-induced RA mice, there were increased ankle joint volumes and clinical scores (p<0.01). The concentration of TNFα was significantly increased from 3 to 7 weeks after immunization. Micro-CT images showed trabecular bone destruction, pannus formation, and subchondral region destruction in RA mice. OA among aged mice showed narrowed joint spaces and breakdown of articular cartilage. This study suggests that a careful therapeutic intervention between RA and OA is required, and it should be based on morphological alteration of bone and joint.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animals
  Induction of RA and Measurements
  Tissue Collection
  Analysis of Serum TNFα Concentration
  Micro-Computed Tomography
  Statistical Analysis
 RESULTS
  Characterization of Collagen-Induced RA Animals
 DISCUSSION
 REFERENCES

키워드

저자

• Yunkyung Hong [ Department Rehabilitation Science in Interdisciplinary PhD Program, Inje University, These authors contributed equally. ]
• Palaksha Kanive Javaregowda [ Cardiovascular & Metabolic Disease Center, College of Biomedical Science & Engineering, These authors contributed equally. ]
• Sang-Kil Lee [ Cardiovascular & Metabolic Disease Center, College of Biomedical Science & Engineering ]
• Sang-Rae Lee [ National Primate Research Center, Korea Research Institute of Biotechnology ]
• Kyu-Tae Chang [ National Primate Research Center, Korea Research Institute of Biotechnology ] Corresponding author
• Yonggeun Hong [ Department Rehabilitation Science in Interdisciplinary PhD Program, Inje University, Cardiovascular & Metabolic Disease Center, College of Biomedical Science & Engineering, National Primate Research Center, Korea Research Institute of Biotechnology ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636