Earticle

초록

영어

Bone morphogenetic proteins (BMPs), which belong to the transforming growth factor (TGF)-β superfamily, can uniquely induce ectopic bone formation. BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It’ involved also in cardiac cell differentiation and epithelial to mesenchymal transition. Recombinant human BMP-2 (rhBMP-2) on an absorbable collagen sponge has been shown to be a safe and effective replacement for iliac crest bone graft when used with a threaded fusion device in anterior lumbar interbody arthrodesis. Insect cell protein expression system is considered to have several advantages in high capacity, flexibility, and similar human glycosylation pattern. For these reasons, the insect cell culture with baculovirus transfection is a useful system for expression of mammalian recombinant protein. hBMP-2 was expressed in insect cell baculovirus system. The cDNA fragment encoding hBMP-2 was amplified for insertion into baculovirus expression vector, pFastBacTMHT. The hBMP-2 expression cassette was inserted into a parent bacmid in DH10Bac (E. coli). The isolation recombinant bacmid was transfected to Sf9 insect cell. The expression of hBMP-2 from the transfected cell was confirmed with immunoblot analysis. Furthermore, we will validate the insect cell expression system as an efficient production reactor for hBMP-2.

키워드

저자

• Jung-Hwan LEE [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea. ]
• Deuk-Soo KIM [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea. ]
• Ying-Xue SHAO [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea. ]
• Jun-Sik YOON [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea. ]
• Se-Ra PARK [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea. ]
• Yong-Seok LEE [ Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje University, Busan, Korea. ]
• Yeon Soo HAN [ Department of Applied Biology, College of Agriculture and Life Science, Chonnam National University, Gwangju, Korea. ]
• Ki-sung KO [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576