Earticle

초록

영어

Potent antimicrobial substances having immuno-modulatory activity were isolated from a Korean soil actinomycetes. These compounds were purified by reverse-phase HPLC with 60% acetonitrile linear gradient. Obtained three compounds showed antimicrobial activity especially against gram positive bacteria including VRSA. MICs of the compounds against 16 species were determined using the agar dilution method. Antimicrobial activity of these compounds were stable in pharmaceutical processing conditions e.g. against heat (up to 90 ℃/ 4 hr, 121℃ /15 pound and 15min), freezing (-20℃) and pH treatment (pH 3-9). Broth agar dilution plate containing CS392s were pre-incubated at 37°C for 0–2 h before inoculation with control strains. These compounds which have strong anti-VRSA (and VRE) showed immuno-modulatory activities. They dose dependently inhibited NO production and inducible NO synthase (iNOS) expression in RAW 264.7 macrophages activated with lipopolysaccharide (LPS). To investigate the signaling pathway for NO inhibition by these compounds, we examined nuclear factor-κB (NF-κB) translocation in RAW 264.7 cells. We concluded that these compounds inhibit the synthesis of pro-inflammatory cytokines and suppress LPS-induced NF-κB activation in RAW 264.7 cells.

키워드

저자

• Jaya Ram SIMKHADA [ Department of Pharmacy, Chosun University, Kwangju 501-759, Republic of Korea. ]
• Yun Hee CHOI [ Department of Pharmacy, Chosun University, Kwangju 501-759, Republic of Korea. ]
• Jin Cheol YOO [ Department of Pharmacy, Chosun University, Kwangju 501-759, Republic of Korea. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576