Earticle

초록

영어

Hydroxy fatty acids can be used as starting materials of resin, waxes, nylons, plastics, lubricants, biopolymer, biodiesel, and additives in coatings and paintings. The recombinant E. coli was used to convert oleic acid to 10-hydroxy stearic acid in order to produce hydroxy fatty acids. The converted 10-hydroxy stearic acids were isolated and purified through biochemical process. The recombinant E. coli was cultivated in Luria-Bertani medium at 37℃, 200rpm. When the OD600nm of the culture reached 0.6, 0.1 mM of IPTG was added. The culture was incubated in the condition of shaking at 150rpm, 16℃ for 12hrs. Tris-HCl buffer was used for the conversion of oleic acid into 10-hydroxy stearic acid at 30℃, pH 7.5. The converted 10-hydroxy stearic acids were tested to find the optimal extraction solvent, solvent ratio and extraction times. 10-Hydroxy stearic acids were extracted better when used ethyl acetate, chloroform, ethyl ether, and hexane. The efficiency of recovery is higher when extraction process is proceeded twice or third times. Also, it is more efficient when the ratio between culture medium and solvent is same.

키워드

저자

• Hyun-Ju KIM [ R & D center, Inwoo Corporation. ]
• Jae-Sang BAE [ R & D center, Inwoo Corporation. ]
• Cheol-Hee KANG [ R & D center, Inwoo Corporation. ]
• Byung-Hee CHUN [ Department of Chemical & Biological Engineering, Korea University. ]
• Jin-Byung PARK [ Department of Food Science and Engineering, Ewha Womans University. ]
• Deok-Kun OH [ Department of Bioscience and Biotechnology, Konkuk University. ]
• Seong-Wook KONG [ R & D center, Inwoo Corporation. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576