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(-)-α-Bisabolol Suppresses Inflammation-associated Gene Expression through Inactivation of Activator Protein-1 (AP-1) and NF-κB Pathway in RAW264.7 Cells

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2011년도 한국생물공학회 춘계학술발표대회 (2011.04)바로가기
  • 페이지
    pp.269-269
  • 저자
    Seung-Beom KIM, Eunsun JUNG, Jongsung LEE, Deokhoon PARK
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A145099

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원문정보

초록

영어
(-)-α-bisabolol is a sesquiterpene alcohol found in the oils of chamomile and other plants. (-)-α-bisabolol has been widely used in dermatological and cosmetic formulations as an anti-inflammatory agent. But so far there were no studies that show the inhibitory mechanism of (-)-α-bisabolol in inflammation-associated gene expression. Therefore, this study was designed to investigate the anti-inflammatory effect of (-)-α-bisabolol and its mechanisms of action. Among many pro-inflammatory mediators, we found that (-)-α-bisabolol inhibited LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW264.7 cells. In addition, in iNOS and COX-2 promoter luciferase assays, (-)-α-bisabolol reduced LPS-induced activation of iNOS and COX-2 promoters. Protein levels of iNOS and COX-2 were also reduced by (-)-α-bisabolol in a concentration-dependent manner. These results suggest that (-)-α-bisabolol exerts anti-inflammatory effects by downregulating expression of iNOS and COX-2.In order to elucidate inhibitory mechanisms of (-)-α-bisabolol on expression of iNOS and COX-2, we investigated effects of (-)-α-bisabolol on LPS-induced activation of AP-1 and NF-kB elements which exist in the promoter of iNOS and COX-2 genes. LPS-induced activation of AP-1 and NF-kB promoters was significantly reduced by (-)-α-bisabolol, suggesting that AP-1 and NF-kB promoters are involved in (-)-α-bisabolol effects. To further confirm this, ELISA for phospho-IkBa and Western blot for phospho-p42/44mapk, phospho-p38mapk, and phospho-JNK were performed. (-)-α-bisabolol reduced LPS-induced phosphorylation of IkBa. In addition, while LPS-induced phosphorylation of p42/44mapk and p38mapk was attenuated by (-)-α-bisabolol, significant change in the level of phosphorylated JNK was not observed. Collectively, our results indicate that (-)-α-bisabolol exerts anti-inflammatory effects by downregulating expression of iNOS and COX-2 genes through the inhibition of NF-kB and AP-1 (p42/44mapk and p38mapk) signaling pathway and suggest that (-)-α-bisabolol may be introduced for the treatment of inflammatory diseases.

키워드

Activaotr protein-1 Nuclear factor kappa B iNOS COX-2 (-)-a-bisabolol

저자

  • Seung-Beom KIM [ Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Sungnam City, Gyunggi Do, Republic of Korea. ]
  • Eunsun JUNG [ Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Sungnam City, Gyunggi Do, Republic of Korea. ]
  • Jongsung LEE [ Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Sungnam City, Gyunggi Do, Republic of Korea. ]
  • Deokhoon PARK [ Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Sungnam City, Gyunggi Do, Republic of Korea. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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