Earticle

초록

영어

We constructed an HPLC-type system suitable for quantitative analysis that enables base sequence- and Tm-dependent DNA oligomer separation using a small open tubular capillary column (300 mm×100 μm I.D.). The DNA oligomers with an amino group at the 5'-end (used as probes) were immobilized on the inner silica surface of the capillary column which had been sequentially treated with APTS (3-aminopropyltriethoxysilane), BMS (butyltrimethoxysilane), and DSG (disuccinimidylglutarate). We succeeded in separating complementary and non-complementary DNA oligomers in specific and quantitative modes. We also designed a temperature gradient strategy for efficient separation of target DNA oligomers. Using a column carrying two different probes with similar Tm values, their complementary target DNA oligomers were also separated and detected. The developed DNA open tubular capillary column system developed in this study could be further improved and used for the quantitative analysis of DNA or mRNA samples.

키워드

저자

• Seung Pil PACK [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700. ]
• Eui Kyoung JANG [ Institute of Advanced Energy, Kyoto University, Kyoto, 611-0011, Japan. ]
• Chanhee CHO [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700. ]
• Ki Baek YEO [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700. ]
• Kiha MIN [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700. ]
• Keisuke MAKINO [ Institute of Advanced Energy, Kyoto University, Kyoto, 611-0011, Japan. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576