Earticle

초록

영어

A biosensor based on the localized surface plasmon resonance (LSPR) response of a single Au nanoparticle was fabricated for the highly sensitive detection and quantification of a specific cancer biomarker. The spectral position changes of single Au nanoparticles induced by the interaction of adsorbates and target analytes were effectively utilized as sensing tools. The LSPR responses of single Au nanoparticles were obtained by tracking the wavelength shift of the corresponding resonant Rayleigh-light-scattering spectra via dark-field microspectroscopy. Using prostate specific antigen as a model, an LSPR λmax shift of about 2.75 nm was recorded by a primary immunoresponse corresponding to 0.1 pg/mL of the target antigen. The sensitivity of the immunoassay can be substantially enhanced, however, by a sandwich strategy. A PSA polyclonal antibody was used as an amplifying agent in the strategy. As a result, the linear dynamic range of the sensing platform was determined to be within the concentration range of 10-4 to 0.1 ng/mL and a detectable minimum concentration of 0.1 pg/mL was identified, with an LSPR λmax shift of about 4.96 nm. The results indicate that the aforementioned approach can significantly contribute to the fabrication of ultrasensitive biosensors, allowing the quantitative analysis of cancerassociated proteins.

키워드

저자

• JONG UK LEE [ Dept. of chemical and biological engineering, Korea University, Seoul 136-701. ]
• SANG JUN SIM [ Dept. of chemical and biological engineering, Korea University, Seoul 136-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576