Earticle

초록

영어

Polydiacetylene (PDA) liposomes which possess unique properties and that properties allow liposomes to change color and emit fluorescence in response to stimulus such as temperature, antibody-antigen interaction, variations in pH, mechanical stress and organic solvent. This material has been extensively studied as signal transducers in biosensor applications. In this research, we demonstrated an antibody-based biosensor using PDA liposomes for detection of hIgE. Target hIgE chemically bound to antibodies-immobilized PDA liposomes and the fluorescence responses were a little bit increased depending on target protein concentration. On the other hand, in case of the addition of gold nano particles-conjugated polyclonal antibody on the liposomes, fluorescent responses dramatically increased depending on target protein concentration. As the results, we could confirm that gold nano particles-conjugated polyclonal antibody affect fluorescent signal amplification of PDA liposomes chip. This strategy can be useful to detect proteins of ultra low concentration.

키워드

저자

• Sang Ho WON [ Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, South Korea. ]
• Sang Jun SIM [ Nano-optics and Biomolecular Engineering National Laboratory, Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, South Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576