The combination of Localized Surface Plasmon Resonance (LSPR) with interferometry using gold-capped porous anodic alumina (PAA) layer chip for the detection of C-reactive protein (CRP) antibody-antigen interactions is demonstrated. The combination of LSPR with interferometry on a gold capped PAA layer chip enables two important aspects of optical sensing systems, the shift in λmax and the increment in the absorbance intensity in a new and highly sensitive format. The excitation of the optical characteristics and the detection was performed using only one optical fiber, which made our device user-friendly and suitable for the development of hand-held diagnostic devices. For the fabrication of PAA layer chip, we developed a two-step anodizing method. The porous portion of PAA layer chip consisted of close-packed hexagonal inter-pores, each with a central straight nano-pore perpendicular to the surface of Al surface. Diameters of the nano-pore and the cell were about 60 nm (1.2X1010pores/cm2) and 120 nm, respectively. Then, PAA layer chip surface was deposited with gold by using thermal evaporator. The CRP antibody immobilization was carried out with the functional SAM on the surface of gold-capped PAA layer chip. For the measurement of CRP antibody-antigen interactions, small volumes of label-free CRP antigen were applied onto the surface of gold-capped PAA layer chip. Subsequently, evaluation of optical properties was carried out. In the presence of CRP antigen, the change of absorbance intensity in the interferometric pattern and a significant bathochromic shift was observed.
Tae Jung PARK [ BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. ]
Do-Kyun KIM [ BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. ]
Sang Yup LEE [ Department of Chemical & Biomolecular Engineering (BK21 program), Department of Bio&Brain Engineering, Department of Biological Sciences, and Bioinformatics Research Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회