Earticle

초록

영어

The recombinant bioluminescent bacteria which contained different promoters fused with either vibrio fisheri lux or bacterial thermorhabidus lux genes can emit bioluminescence upon the transcription initation at its proper temperatures either 30℃ or 37℃. We have evaluated total 29 different recombinant bioluminescent bacteria with gamma-ray irradiation in order to study their specific responses which act upon different stresses such as DNA-damage, oxidative damage, cell-wall damage, hydroxyl-radical damage, benzoic acid damage, and so on. Using 96 well-plates with different gamma-ray doses, we found that some bacteria containing DNA-damage sensitive promoters such as recA, SbmC, SulA, RecN have shown the sensitive bioluminescent from above the specific gamma-ray dose, while other DNA-damage sensitive bacteria containing NrdA or AlkA and other stress-response bacteria have not shown significant responses in their bioluminescence. It is expected that these distinct responses to gamma-ray irradiation can be used as biosensors for environment or space applications.

키워드

저자

• Hyun LEE [ College of Life sciences and Biotechnology, Korea University, Seoul, 136-701, Korea. ]
• JiHoon KIM [ College of Life sciences and Biotechnology, Korea University, Seoul, 136-701, Korea. ]
• JooMyung AHN [ College of Life sciences and Biotechnology, Korea University, Seoul, 136-701, Korea. ]
• YoungHoon JI [ Korea Institute of Radiological & Medical Sciences, Seoul, 139-706, Korea. ]
• ManBock GU [ College of Life sciences and Biotechnology, Korea University, Seoul, 136-701, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576