Earticle

초록

영어

The promoter activity is a key factor in a cell for the active growth of living things and mass production of useful materials. To investigate the promoter activity of eukaryotic cell, in-vivo transcription has been usually used. However, for the faster monitoring of genetic information processing, in-vitro transcription process is essentially needed, because eukaryoticcell grows up slowly. Here we compared the several promoters activities by a simplified microalgal in-vitro transcription. RNA polymerase and the other cofactors were seperated from the chlamydomonas's cell lysate using MNP-DNA conjugates. Subsequently, concentration of mRNA produced by in-vitro transcription was measured by Qubit fluorometer. As a results, we could observe the changes in amounts of mRNA depends on the kinds of promoters. Thus, the best promoter could be selected. This system allows the fast,easy and economical analysis of promoter activity of microalgal under in-vitro transcription condition.

키워드

저자

• Min Sun SONG [ Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, South Korea. ]
• Seung Phill CHOI [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, South Korea. ]
• Sang Jun SIM [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, South Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576