The highly sensitive detection of norovirus and pathogenic bacteria in the food samples is of great importance because food poisoning outbreak has been wide occurred worldwide. For the molecular detection of a pathogen in the food samples, sample preparation procedures are required. Various methods to isolate a pathogen from the real samples have been reported. These methods, however, don’ fit for the micro total analytical system. Here, we’e developed the simple concentration method of norovirus from the oyster sample for the nucleic acid based detection of norovirus. The adsorption of norovirus on the solid surface was investigated with additives (ions, surfactants, pH) and surface characters (hydrophobicity and charge). As a result, we could isolate and concentrate norovirus from oyster samples (contains E.coli and protein), which results in that high sensitive detection of norovirus in low concentration in the oyster sample [101 PFU/a piece, PCR-calculated concentration] was achieved. Acknowledgment: This subject is supported by Ministry of Environment of the Republic of Korea as “he Eco-Technopia 21 project”and by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. 20100020657)).
키워드
norovirusadsorptionlow concentrationoyster
저자
Ji Yeong WON [ Department of Chemical and Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742. ]
Ja Yeon KIM [ Gachon Bio-nano Research Institute, Kyungwon University, Bokjung-dong, Sujung-gu, Sungnam 461-701. ]
Junhong MIN [ College of Bionano technology, Kyungwon University, Bokjung-dong, Sujung-gu, Sungnam-si, Gyunggi-do 461-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회