The highly sensitive detection of pathogen in the soil samples is great importance because food poisoning outbreak has been occurred mostly from the pathogen infection of the soil. To detect low concentration of pathogen in a soil using PCR amplification, the removal process of humic substances is required because they are known as an inhibitor to PCR amplification. Various methods to remove humic substances in soil samples for nucleic acid based detection of pathogen have been developed. However, these methods don’ suitable the micro total analytical system. In this study, we’e developed the simple separation and concentration method of pathogen from the soil samples including humic acid for the nucleic acid based detection of pathogen. The humic acid was removed from the soil samples by optimal solution chemistry and physical adsorption. As a result, we could detect pathogen in low concentration in the soil sample included humic acid [<102 c.f.u/soil 10g]. Acknowledgment: This subject is supported by Ministry of Environment of the Republic of Korea as “he Eco-Technopia 21 project”ò and by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. 20100020657)).
키워드
humic acidseparationrt-PCRinhibitorpathogen
저자
Hee Yeon KIM [ Department of Chemical &Biomolecular Engineering, Kyungwon University, Bokjung-dong, Sujung-gu, Sungnam-si, Gyunggi-do 461-701. ]
Ji Yeong WON [ Department of Chemical & Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742. ]
Junhong MIN [ College of Bionano technology, Kyungwon University, Bokjung-dong, Sujung-gu, Sungnam-si, Gyunggi-do 461-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회