In this study, to identify proteins involved in tumor progression and to deeply understand mechanism underlying melanoma metastasis, comparative proteome analysis on two melanoma cell strains with low and high metastatic potentials, WM793 and 1205LU, respectively, was performed using a combined three-dimensional difference in gel electrophoresis (3-D DIGE) technology [1,2]. The majority of protein level changes are closely associated with many aspects of the pathophysiology of cancers, such as cell death and growth, or tumorigenesis. In addition, cellular pathways related in oncogenes (JNK, c-myc, and N-myc) and tumor suppressor genes (p53 and TGF-β) were found to be prevalent metastatic melanoma progression by the Ingenuity Pathway Analysis. These networks showed substantial overlap and connectivity with the main biological theme of cell death and cancer. Thus the use of more comprehensive proteins profiling to analyze genetically very similar melanoma cell lines can contribute to the identification of novel metastatic biomarkers and provide new insights into the protein pathways involved in melanoma metastasis. [This work was supported by the Basic Science Research Program (2010-0008826) and Converging Research Center Program (2009-0093652) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology].
키워드
2-D difference in gel electrophoresis (2-D DIGE)Metastatic melanomaMicroscale solution isoelectric focusing (MicroSol-IEF)Potential biomarkersProtein pathways
저자
Mee-Jung HAN [ Dept. of Biomolecular and Chemical Engineering, Dongyang University, Gyeongbuk 750-711. ]
David W. SPEICHER [ The Wistar Institute, Philadelphia, PA 19104, USA. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회