A human hybrid cell line named F2N78 was developed by somatic fusion of human embryonic kidney (HEK293) cells and Namalwa cells. Human cells have a human-like glycosylation profiles, so that they should be efficient for the production of human therapeutic proteins considering efficacy and stability. As a result of cell fusion, F2N78 cell line has the characteristics of both HEK 293 and Namalwa cells, such as high transfection efficiency, ease of suspension culture, and continuous expression of EBNA1 gene by EBV genome. In order to be used in commercial processes, development of optimal media for the growth and transfection of this novel host cell line is necessary. For that reason, development of an optimized medium for F2N78 cell line was performed in this study. Various media such as EXCELL 293, CD OPTI CHO, MPC002 were compared for the growth and transfection of F2N78 as well as HEK293. It was found that MPC002 medium provided the best results for growth of F2N78 cells and the modification and optimization of MPC002 medium will be carried out further.
키워드
human hybrid cell lineF2N78media screening
저자
Joon-Serk SEO [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
Keun-Hee PARK [ Celltrion, Incheon, 406-840, Korea. ]
Dong-Il KIM [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회