Animal cells are sensitive to both DO and shear stress. First of all, we investigated O2 transfer rate and shear stress of bubble column bioreactors. In the reactors, kLa increased with aeration rate and decreased with the size of bubbles. As far as the bubble size is concerned, small bubbles showed higher O2 transfer than large bubbles. Small bubbles, however, caused shear stress problem that damaged CHO cells. Similar phenomenon was observed in shaking flasks. In well plates or T-flasks, O2 transfer rate was higher in twist-shaking than in tilted-shaking. However, cell growth and viability of twisted flasks were lower than that of tilted ones. At the same time, it was observed that cells were aggregated more in twisting than in tilting. In order to investigate the shear stress marker of CHO cells, 2-D analysis was performed. HSP90-β and G protein could be included in several proteins that might be used as anchorage or shear index proteins. Relationship between oxygen transfer efficiency and shear stress will be presented in this paper.
키워드
CHO cellBubble column bioreactorOxygen TransferShear stress2-D analysis
저자
Ye Young YOO [ Dept. of Life Sciences and Biotechnology, Korea University, Seoul, 136-701. ]
Juno JANG [ Dept. of Life Sciences and Biotechnology, Korea University, Seoul, 136-701. ]
Ik-Hwan KIM [ Dept. of Life Sciences and Biotechnology, Korea University, Seoul, 136-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회