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포스터 발표 : 동물 및 식물세포공학
Integrated Vector System for Phage Display and Mammalian Expression of Antibody Library
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- 발행기관
- 한국생물공학회 바로가기
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- 간행물
- 한국생물공학회 학술대회 바로가기
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- 통권
- 2011년도 한국생물공학회 춘계학술발표대회 (2011.04)바로가기
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- 페이지
- pp.251-251
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A145039
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Phage display has now become well established technology and enabled construction and selection of large size of antibody library in vitro. Millions of candidate clones are commonly obtained following rounds of phage-displayed library panning, and labor-intensive expression of those selected clones is required for secondary screening to identify functionally positive clones with therapeutic potential. Here, we designed integrated vector system for phage display and mammalian expression for rapid expression and characterization of selected antibody clones from library. We took advantage of mammalian/bacterial hybrid signal peptide for mammalian expression which accommodates compatible restriction enzyme sites with bacterial signal peptide, which enables the direct transfer of even unknown antibody sequences from phagemid to mammalian expression vector. Its utility was demonstrated for antibody library selection against several targets. First, the vector system was applied for the construction and selection of antibody library recognizing cancer-related aberrant glycosylation of human tissue inhibitor of metalloproteinase-1 (TIMP-1). The selected antibody clones could track down subtle change of glycan structure on the TIMP-1. Then the system was used for the selection of antibodies recognizing membrane proteins on cell surface. Both TM4SF5 and CD9 belong to tetraspanin superfamily and are reported as candidate cancer therapeutic targets. TM4SF5 was prepared as recombinantly expressed Fc-fusion form and used for library panning and selection. On the contrary, CD9 was expressed on cell surface as native form, and the recombinant cell line was directly used for cell panning and selection. For both targets, we could successfully isolate and identify specific binders. Their binding characteristics were confirmed by using ELISA and FACS. Further, we investigated their therapeutic potentials using cell-based assays.
키워드
phage display antibody Library저자
간행물 정보
발행기관
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- 발행기관명
- 한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
- 설립연도
- 1984
- 분야
- 공학>생물공학
- 소개
- 이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
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- 간행물명
- 한국생물공학회 학술대회
- 간기
- 반년간
- 수록기간
- 1985~2013
- 십진분류
- KDC 476 DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회
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