Earticle

초록

영어

Classical swine fever virus causes significant economical losses in pig industry in many countries. So the development of porcine cholera marker vaccine is urgently required. The baculovirus-insect cell system has been established as a powerful technology for the production of high value recombinant proteins. Baculovirus is safe virus because it has a limited host range, and it produces heterologous recombinant protein under polyhedrin promoter. Insect cells are an attractive host for recombinant protein expression via infection with a genetically modified baculovirus as this system allows for high levels of production of proteins that are functionally similar to the native protein. So insect cell-baculovirus vector system was used in this study for producing porcine cholera maker vaccine. The effects of agitation speed and infection time, pH on marker vaccine production were investigated in each case of individual cell line. As a result, the highest productivity of porcine cholera marker vaccine achieved was 61.26 mg/L at the condition of 1 day infection time with sf21 cells. And highest productivity of porcine cholera marker vaccine achieved was 169.29 μ g/mL at the condition of 100 rpm with sf9 cell. The productivity of 194.74 mg/L was obtained by the sf9 cell in case of pH shift from 6.1 to pH 6.5.

키워드

저자

• Jung-Min CHOI [ Dept. of Medical Biotechnology, Kangwon National University, Chuncheon, 200-701. ]
• Je-Sin WOO [ Dept. of Medical Biotechnology, Kangwon National University, Chuncheon, 200-701. ]
• Chang-Jin LEE [ Dept. of Medical Biotechnology, Kangwon National University, Chuncheon, 200-701. ]
• Yeon-Ho JEONG [ Dept. of Medical Biotechnology, Kangwon National University, Chuncheon, 200-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576