Earticle

초록

영어

Platelet rich plasma (PRP) is increasingly used in the treatment of wound healing. We have developed heparin-conjugated fibrin (HCF) which can exert controlled release of various growth factors contained in PRP. Here, we hypothesized HCF can deliver growth factors contained in PRP for a long period and enhance the wound healing effect of PRP. PRP was prepared by centrifuging whole blood. Full-thickness (2.0 x 2.0 cm) wounds were created on the dorsum of athymic mice. HCF mixed with PRP and thrombin was applied at the wound sites. No treatment, application of PRP with thrombin, PRP with fibrinogen and thrombin served as controls. Skin regeneration was evaluated by histological and immunohistochemical analyses. Initial burst release and release period dramatically differ depending on the types of the delivery system in vitro. At 12 days after injury, PRP with HCF group showed complete epithelialization of the wound compared to the other groups. The macroscopic wound sizes of PRP with HCF group were statistically smaller than the other groups at 12 days. HCF could be useful for delivery of various heparin-binding growth factors contained in PRP to promote wound healing.

키워드

저자

• Jung-Youn SHIN [ School of Chemical and Biological Engineering, Seoul National University, San 56-1, Silim-dong, Gwanak-Gu, Seoul 151-744. ]
• Hee Seok YANG [ Department of Bioengineering, Hanyang University, 17 Hangdang-Dong, Sungdong-Gu, Seoul 133-791. ]
• Jaehoon SHIN [ School of Chemical and Biological Engineering, Seoul National University, San 56-1, Silim-dong, Gwanak-Gu, Seoul 151-744. ]
• Suk Ho BHANG [ School of Chemical and Biological Engineering, Seoul National University, San 56-1, Silim-dong, Gwanak-Gu, Seoul 151-744. ]
• Byung-Soo KIM [ School of Chemical and Biological Engineering, Seoul National University, San 56-1, Silim-dong, Gwanak-Gu, Seoul 151-744. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576