Adipose tissue-derived stem cells (AT-MSCs) can be extracted from adipose tissue and obtained by a less invasive method and in larger quantities compared with bone marrow–erived MSCs. And many studies have demonstrated that AT-MSCs have differentiate potential to neuronal cells by treating mechanical and chemical methods. The aim of this study is to investigate the effects of sonic vibration (SV) on AT-MSCs and to explore their neuronal differentiation potential. We decided AT-MSCs were exposed to sonic vibration during 4 days at 40 Hz, 1.0 V. After stimulation, we found the differences between sonic vibration exposed group and on-exposed group in terms of cell morphology and RT-PCR electrophoresis products. In the sonic vibration exposed group, RT-PCR indicated that expression level of neuronal markers including NeuroD1, Map2 and NF-L is significantly higher than non-exposed group. So these results suggest that sonic vibration affects MSCs to induce the neuron-like stem cells. And adipose tissue may be an alternative source of stem cell therapy for nervous system injury.
키워드
Sonic VibrationAdipose tissueMesenchymal stem cellNeuronal differentiation
저자
Yun-Kyong CHOI [ Dept. of Medical Biotechnology, Dongguk University, Seoul, 100-273. ]
Hyun-Jin CHO [ Dongguk University Research Institute of Biotechnology, Seoul, 100-273. ]
Jung-Keug PARK [ Dept. of Medical Biotechnology, Dongguk University, Seoul, 100-273. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회