Earticle

초록

영어

Cross-linking of collagen using the reactive hydroxy-, amino- and carboxygroups on the collagen molecule has been described previously. Recently, non-toxic cross-linking agent has been described based on water-soluble carbodiimide and N-hydroxysuccimide (EDC-NHS). Also, cross-linking reagents, consisting of natural polymers has a lot of research. In this study Genipin (GP) and Grape Seed Extract (GSE), a significantly less cytotoxic cross-linking agent compared to EDC-NHS, was used to cross-link collagen-CS scaffold for jurkat cell. In other words, we evaluated the effects of cross-linking reagents on the cell growth and cytokine production in three-dimensional culture. These reports showed that cell growth in the EDC-NHS was significantly higher than that of the other groups. Scanning electron microscopy (SEM) revealed the porous surface of each scaffolds maintained their spherical morphology. In addition, cytokine amount through IFN-r, TNF-a, IL-2, and IL-6 assay, that GP and GSE revealed higher than EDC-NHS. GP showed high cross-linking ability, along with toxicity and immune response, respectively. GSE compared with the GP or the EDC-NHS more toxic to the cell itself, given the three-dimensional scaffolds for the culture of cross-linking agent was inappropriate at this time. Thus, cross-linking of the cross-linked scaffold on each culture tests, impact on cells and cytokines secreted by the amount of EDC-NHS creatures of the lowest toxicity was found.

키워드

저자

• Mi Na KANG [ Dept. of Medical Biotechnology, Dongguk University, Seoul, 100-715. ]
• Young Kwon SEO [ Research Institute of Biotechnology, Dongguk University, Seoul, 100-715. ]
• Jung Keug PARK [ Dept. of Medical Biotechnology, Dongguk University, Seoul, 100-715. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576