Earticle

초록

영어

The combination of viral gene delivery and key features of biomaterial scaffolds that control viral delivery in a controlled manner greatly enable tissue engineering applications. In this study, adeno-associated virus (AAV), which have safety and efficient gene delivery capability in human gene therapy as therapeutic vectors, was encapsulated by electrospinning blended mixtures of elastin-like polypeptides (ELPs) and poly (e-caprolactone) (PCL) and was employed to transduce fibroblasts adhered on the scaffolds. Combinatorial interactions between ELP and PCL chains with varying ratios significantly altered wettability, elastic modulus and strain of the ELP/PCL composites. Then this system was useful tools to mediate controlled release of AAV vectors and efficient cellular transduction on the fibrous scaffolds. The capacity of ELP/PCL composites to modulate the controlled release of AAV-mediated gene delivery for subsequent high-efficiency cellular transduction will provide tremendous opportunities for a variety of tissue engineering applications.

키워드

저자

• Slgirim LEE [ Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Korea, 120-749. ]
• Jung-suk KIM [ Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Korea, 120-749. ]
• Hun Su CHU [ School of Chemical and Biological engineering, Seoul National University, Seoul, Korea. ]
• Jong-In WON [ Department of Chemical Engineering, Hongik University, Seoul, Korea. ]
• Jae-Hyung JANG [ Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Korea, 120-749. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576