Earticle

초록

영어

In this study we are willing to recognize the effect of collagen scaffold contained with HA(Hyaluronic acid) for dental tissue engineering. The type I atelocollagen was mixed with 0%, 10%, 25% of HA. Then, the solutions were using to make collagen-HA sponge scaffolds. The diameter of scaffolds was 3cm. Scaffolds were crosslinked with 0.02% carbodiimide and lyophilized for 48 h. And they were sterilized by γ- irradiation at 10 KGy. DPSCs(dental pulp stem cells) were seeded into scaffolds at a density of 5x105 cells/scaffold and cultured for 3weeks in growth medium. Then, they were cultured for 4weeks in differentiation medium and were transplanted in the nude mouse. The biopsy was processed at 8weeks. Col I and fibronectin, osteocalcin, osteopontin, osteonectin, osteoprotegerin levels in the culture were greatest in the 10% of HA. The calcification and the revelation of ostecalcin, osteopontin, osteonectin, and osteoprotegerin were better made in the collagen-HA sponge scaffold contained with 10% of HA. We could make collagen sponge scaffold contained various concentration of HA. Among them, 10% of HA was the most effective for osteogenesis. It will be a suitable substrate as biomaterial for bone tissue.

키워드

저자

• Mi-Jung HAN [ Dongguk University. ]
• Young-Kwon SEO [ Dongguk University Research Institute of Biotechnology. ]
• Hee-Hoon YOON [ Dongguk University Research Institute of Biotechnology. ]
• Kye-Yong SONG [ Department of Pathology, Chung-Ang University. ]
• Jung-Keug PARK [ Department of Medical Biotechnology, Dongguk University. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576