2,3-butanediol (2,3-BD) is a very useful chemical, which is used as a solvent, a liquid fuel, and a precursor of many synthetic polymers. A 2,3-BD-producing bacterium, which was identified as Raoultella sp. B6, was isolated from an oil-contaminated soil sample. The isolate could produce 2,3-BD using various carbon sources. The effects of initial glucose concentration, initial pH, and temperature on 2,3-butanediol production were investigated using flask test. These parameters influenced the productivity and yield of 2,3-BD, and the production of the other fermentation products such as succinate, lactate, acetate, formic acid, acetic acid, and ethanol. The final 2,3-BD concentration was proportional to the initial glucose concentration. However, the productivity is inversely related to the initial glucose concentration. The growth rate and 2,3-BD production was the highest at neutral pH. The bacterium produced 2,3-BD at the start of the cell growth at 30 ℃, whereas it produced 2,3-BD in the middle of exponential phase at 25℃. Nevertheless, the highest concentration of 2,3-BD was the same in both cases. Based on these results, fed-batch culture was performed with or without aeration. This experiment showed that aeration has a positive effect on the cell growth and 2,3-BD production.
키워드
23-Butanediol23-BDRaoultella
저자
Taeyeon KIM [ Clean Energy Center, Korea Institute of Science and Technology (KIST) Seoul 136-791. ]
Byoung-In SANG [ Clean Energy Center, Korea Institute of Science and Technology (KIST) Seoul 136-791. ]
Youngsoon UM [ Clean Energy Center, Korea Institute of Science and Technology (KIST) Seoul 136-791. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회