Earticle

초록

영어

Mercuric compounds are very toxic. There are many reports indicating its genotoxic potential in a variety of organisms including humans and aquatic species [1]. Inorganic mercury occurs in numerous forms. The most common include mercuric chloride (HgCl2) and mercurous chloride (Hg2Cl2). Mercuric chloride adversely affects the central and peripheral nervous system, the kidney, the eye and reproductive fitness [2]. Meanwhile, earthworms can be used as a bio-indicator of contamination in soil [3]. This study was performed to investigate the acute toxic effects of mercuric chloride in earthworm, Eisenia fetida. The earthworms were exposed to 0 ~ 160 mg/kg of mercuric chloride in the soil for 24 and 48 hours. Then DNA damage and repair was measured with the comet assay from 0 to 72 hours after HgCl2 treatment. The level of DNA damage in the earthworms was shown as the olive tail moment. The DNA tail moment is defined that both the length of tail and the intensity of the DNA in the tail. The results showed that the higher the concentration of mercury was treated, the greater damage and the longer repair time were expressed. This study confirmed that the comet assay applied on earthworm’ coelomocytes may be a useful tool in determining the potential genotoxicity of soil pollutants.

키워드

저자

• Jin Kyu KIM [ Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute, Jeongeup, 580-185. ]
• Tae Ho RYU [ Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute, Jeongeup, 580-185. ]
• Mohammad NILI [ Dawnesh Radiation Research Institute, Barcelona, 08007, Spain. ]
• Kwang-Guk AN [ College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576