Earticle

초록

영어

Insulin, in nature, has a stimulatory effect on microorganisms. This causes speeding up in the sugar metabolism and also triacylglycerol anabolism increasing rapid growth rate and formation of high amount of oil content. On the other hand, we observed in our study that insulin may cause indirect activation of triacylglycerol lipase by making the cell in permanent needs to the energy source. Thus, cells consume all its sources of fuel which is accumulated inside the cells like lipids, proteins and carbohydrates. After studying the effect by using two type of insulin (Humulin 70\30 and insulin human expressed in yeast) in different concentrations on microalgae Chlorella spp., we found that incertain concentrations of insulin (1:2.6 micro unit Humulin 70\30 per ml, 1:3.3 micro unit yeast insulin per ml), algae expressed on growth rate and cell size. This suggests that algae have high optical density and smaller cell size than control. We, therefore, studied the effect of insulin under lipase inhibition by using Triton WR 1339 (Tyloxapol) which was used in different concentrations with and without insulin. We found strong regression in the growth rate with increasing Triton concentration. However, we also recorded that the cell size under the effect of Triton and Triton-insulin was higher than the cell size under the effect of insulin alone and even higher than the control sample. The oil content, also, was higher than the control sample and the cells under just insulin effect.

키워드

저자

• Gyuhwa CHUNG [ Chonnam National University. ]
• Ahmed E. GOMAA [ Chonnam National University. ]
• E.E. HAFEZ [ MUBARAK City for Science and Technology. ]
• Hyun Soo LIM [ Chonnam National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576