Earticle

초록

영어

Jerusalem artichoke, one of the low-requirement sugar crops, is an inexpensive and wildly available non-grain raw biomaterial containing cellulose and hemicelluloses in the stalk and a high content of inulin in the tuber. However, the lignocellulosic component in Jerusalem artichoke stalk reduces the fermentability of the whole plant for efficient bioethanol production. In this study, Jerusalem artichoke stalk was pretreated by diluted acid treatment and then enzymatically hydrolyzed. The optimum pretreatment conditions were at 80°C, 6 h retention time and 4% sulfuric acid solution to dry biomass loading of 20:1 (wt/wt). The cellulose and hemicellulose from the tuber were released into the acidic solution. During enzymatic hydrolysis using Celluclast®1.5L (60 FPU/ g dry biomass) and Novozyme 188 (30 CBU/ g dry biomass), approximately 90% of glucan and xylan were converted to glucose and xylose. It was close to the maximum yield within 48 h of enzyme hydrolysis. Soluble sugars present in the hydrolysate of the stalk were extracted with hot water, and then the extract was combined with the lysate of Jerusalem artichoke tuber for fermentation. Batch simultaneous saccharification and fermentation by Kluyveromyces maxianus, an inulinase activity yeast, was effectively performed and over 10 ml ethanol per 100 ml of the fermented medium was yielded, the ethanol productivity was over 0.35 g ethanol/ g of dried Jerusalem artichoke and over 95% of total sugar was used by the yeast strain. This result showed that combining tuber to the stalk hydrolysate was a useful strategy of whole biomass utilization for effective fermentation of bioethanol production from Jerusalem artichoke.

키워드

저자

• Jang Min PARK [ Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Korea. ]
• Seonghun KIM [ Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Korea. ]
• Jeong-woo SEO [ Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Korea. ]
• Churl Ho KIM [ Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Korea. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576