Pilot-scale optimization of parameters related to dissolved oxygen for mass production of carboxymethylcellulase by recombinant E. coli JM109/DL-3 from rice bran using response surface method
Sang-Un Lee LEE, Wa GAO, You-Jung LEE, Chang-Woo SON, Bo-Kyung KIM, Jin-Woo LEE
언어
영어(ENG)
URL
https://www.earticle.net/Article/A144844
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원문정보
초록
영어
The optimal agitation speed and aeration rate for mass production of carboxymethylcellulase (CMCase) of B. amyloliquifaciens DL-3 by E. coli JM109/DL-3 were established in a 7 L bioreactor using the response surface method. The analysis of variance (ANOVA) of results from central composite design (CCD) indicated that highly significant factor ("probe>F" less than 0.0001) for cell growth and production of CMCase was the aeration rate. The optimal agitation speed and aeration rate for cell growth were 498rpm and 1.4 vvm, whereas those for production of CMCase were 395 rpm and 1.1 vvm. The optimal inner pressure in a 100L bioreactor for cell growth was 0.8 kgf/cm2, whereas that for production of CMCase was 0.6 kgf/cm2. The maximal production CMCase by a recombinant E. coli JM109/DL-3 under optimized conditions in this study was 871.0 U/mL, which was2.4 times higher than that by B. amyloliquifaciens DL-3.
키워드
carboxymethylcellulase (CMCase)B. amyloliquifaciens DL-3E. coli JM109/DL-37 L bioreactor100L bioreactor
저자
Sang-Un Lee LEE [ Dept. of Medical Bioscience of Graduate School of Dong-A University, Busan 604-714, Korea. ]
Wa GAO [ Dept. of Medical Bioscience of Graduate School of Dong-A University, Busan 604-714, Korea. ]
You-Jung LEE [ Dept. of Medical Bioscience of Graduate School of Dong-A University, Busan 604-714, Korea. ]
Chang-Woo SON [ Korea Biosolution Co., Busan 612-021, Korea. ]
Bo-Kyung KIM [ Korea Biosolution Co., Busan 612-021, Korea. ]
Jin-Woo LEE [ BK21 Bio-Silver Program of Dong-A University, Busan 604-714, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회