Earticle

초록

영어

The optimal conditions for production of carboxymethylcellulase (CMCase) of B. amyloliquifaciens DL-3 by E. coli JM109/DL-3 were established using the response surface method. The optimal carbon and nitrogen sources for production of CMCase were found to be rice bran and tryptone, respectively. The analysis of variance (ANOVA) of results from central composite design (CCD) indicated that significant factor ("probe>F"less than 0.0500) for cell growth was rice bran, whereas those for production of CMCase were rice bran and initial pH of the medium. The optimal conditions of rice bran, tryptone, and initial pH for cell growth extracted by Design Expert Software were 66.1 g/L, 6.2 g/L, and 7.2, respectively, whereas those for production of CMCase were 58.0 g/L,5.0 g/L, and 7.1. The optimal temperature for cell growth and production of CMCase were found to be 37℃. The maximal production CMCase by a recombinant E. coli JM109/DL-3 was 487 U/mL.

키워드

저자

• Eun-Jung LEE [ Dept. of Medical Bioscience, Graduate School of Dong-A University, Busan 604-714, KoreaDepartment of Medical Bioscience of Graduate School of Dong-A University, Busan 604-714, Korea. ]
• You-Jung Lee LEE [ Dept. of Medical Bioscience, Graduate School of Dong-A University, Busan 604-714, KoreaDepartment of Medical Bioscience of Graduate School of Dong-A University, Busan 604-714, Korea. ]
• Chang-Woo SON [ Korea Biosolution Co., Busan 612-021, Korea. ]
• Dae-Young JUNG [ Korea Biosolution Co., Busan 612-021, Korea. ]
• JIn-Woo LEE [ BK21 Bio-Silver Program of Dong-A University, Busan 604-714, Korea. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

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  KDC 476 DDC 576